Applications of combinations and concentrations of different phytohormones for the induction of callus, shoot, and root of Cannabis sativa under tissue culture technique
induction of callus, shoot, and root of cannabis sativa
DOI:
https://doi.org/10.47743/jemb-2024-174Keywords:
Cannabis sativa; Murashige and Skoog; indole-3-acetic acid; Kinetin 2,4D; BAP; Kinetin;IAA; IBA.Abstract
Cannabis sativa in vitro cultivation interest has been invigorated/renewed due to health and economic benefits, unconventional strategy, quality plants, and industrial fiber. The in vitro regeneration of cannabis encountered establishment and disinfection aspects globally. The research on plant growth regulator composition, combination, concentrations, decontamination, significant influence on, callus, shoot, and root induction, and regeneration is needed to shed light on desirable, healthy plants for the cannabis industry. The study outcomes indicated some disinfectant duration and concentrations exposure to ex-plant have a toxic impact and reduced growth while 5 % sodium hypochlorite and mercury chloride 0.1-1% are efficient sterile and gave good seed germination. A sufficient increase in callus proliferation was noticed on combination and concentrations of MS (Murashige and Skoog) media treated with 450 µl kinetin +450 ml BAP+450 µl 2,4-D from Cannabis sativa leaves. Substantial influence on shoot survival was recorded on MS media supplemented with increasing 2-3 ml BAP concentration. Maximum rhizogenesis observed on PGR (plant growth regulator) 5ml (indole-3-acetic acid) IAA and 8ml (indole-3-butyric acid) IBA combinations. The study output suggests that in vitro cultivation using phytohormones protocols is applicable and appropriate for cannabis survival and productivity and can be valuable for medical and industrial purposes.
References
Wang, T., et al., Adverse effects of medical cannabinoids: a systematic review. Cmaj, 2008. 178(13): p. 1669-1678.
Roberts, M., Medicinal products through plant biotechnology. Manipulating Secondary Metabolism in Culture, 1988: p. 201-216.
Gagné, V., N. Boucher, and I. Desgagné-Penix, Cannabis roots: therapeutic, biotechnological and environmental aspects. Cannabis and cannabinoid research, 2024.
Monthony, A.S. and A.M.P. Jones, Enhancing Protoplast Isolation and Early Cell Division from Cannabis sativa Callus Cultures via Phenylpropanoid Inhibition. Plants, 2024. 13(1): p. 130.
ÇÖRDÜK, N., et al., A comparative study of the antimicrobial activities of field-grown and in vitro grown plants of Verbascum scamandri Murb. ADVANCED AND CONTEMPORARY STUDIES IN NATURAL SCIENCE AND MATHEMATICS: p. 82.
Rastgoo, S., B. Raju, and B. Satyanarayana, In vitro regeneration from pummelo (Citrus grandis osbeck.) cotyledon-derived callus. Acta horticulturae, 2011(890): p. 139.
Tzatzani, T.-T., K. Dimassi, and I. Therios, Organogenesis of citrus rootstocks using mature explants. Journal of Agriculture and Environmental Sciences, 2018. 7(1): p. 10-15.
Lahiri, K., et al., Rapid and stable in vitro regeneration of plants through callus morphogenesis in two varieties of Mucuna pruriens L.–an anti Parkinson’s drug yielding plant. The Nucleus, 2012. 55: p. 37-43.
Asgher, M., et al., Minimising toxicity of cadmium in plants—role of plant growth regulators. Protoplasma, 2015. 252: p. 399-413.
El-kafie, A., et al., Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L. Journal of Plant Production, 2019. 10(7): p. 495-501.
Gottschling, S., et al., Safety considerations in cannabinoid-based medicine. International journal of general medicine, 2020: p. 1317-1333.
ÖZDEMİR, F.A. and M. TÜRKER, In vitro plant regeneration influence by BAP and IBA in lentils (Lens culinaris Medik). Journal of Applied Biological Sciences, 2014. 8(1): p. 22-27.
Sharma, N.K., M. Kumar, and R. Choudhary, Effect of 2, 4-D, BAP, KN, IAA and IBA on in vitro Regeneration of Ocimum canum Sims–An Important Hoary Basil Plant. International Journal of Agriculture, Environment and Biotechnology, 2013. 6(3): p. 389-396.
Downloads
Published
How to Cite
License
Copyright (c) 2024 muhammad waqar mazhar, sadaf javed
This work is licensed under a Creative Commons Attribution 4.0 International License.
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. The journal allows readers to read, download, copy, distribute, print, search, link to the full texts or use the articles for any other lawful purpose.
The authors are the sole copyright owners of the published articles. The articles are distributed under the CC BY 4.0 license to the readers.
The readers are free to:
Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material for any purpose, even commercially
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
No additional restrictions — you may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Reviewer Recognition Service. Forward your thank you e-mail to 
